Based on some of your comments, and other inspirations, I posted a long page of links and excerpt/fragments covering visible bacteria sources and methods, that might be used for art or easier cheaper faster measurement of test results. Ver 2 july 12, 2016
Table of Contents
1. so the stuff glows under the 'black light' less before oxegen in depleted, then it glows more. Other stuff might glow under the black light so they do stuff like narrow laser and LED lighting and turning it off and seeing how many milliseconds the glow stays on, and non oxygen sensitive dyes can be used as a baseline. $120 of materiels to get into the game
2. $ 350, special broth makes color changing from 'indicator' non pathenogenic ecoli, part of the poop detection industry.
2.5 ONPG broth agent 5g $87, additional broth and spliced bugs unknown.
3. Grow some marine bioluminescent bacteria. $372, hope their cousins cholera, flesh eating sepsis bacteria don’t get into the mix and into our bodies.
4.
Blue is alkaline and Red is acidy using this product chemical of a
bacteria, sort of a litmus test, maybe all I care about is if any
color is produced equals growth.
$354
for the germ plus the broth,
5. ammonia
eating bacteria for aquariums can cause white materiel, but I have
not been able to get it to work sensibly.
6. Consumption of dyes by bacteria, so far have not gotten anything to work, many suggested bacteria are pathogens I don’t want to work with.
Intro...
http://biorxiv.org/content/biorxiv/early/2016/03/05/042499.full.pdf
The
above lays down the problem and the path to a solution which I hope
to lazily accomplish with this
hardware:
http://www.target.com/p/Oval%20Picture%20Frame%20-%20Black%20-%208x10%20-%20Room%20Essentials%E2%84%A2/-/A-17318275&ref=Order_Delivered_Email
http://pharmacyte.com/live-cell-encapsulation/
"Cell-in-a-Box®
encapsulation differ from those produced by others. Our competitors
use substances such as alginate (a seaweed derivative) or chitosan,
whereas our capsules are made principally of cellulose, a bio-inert
material in the human body.”
some
backgrounder and
nomenklatura...
https://en.wikipedia.org/wiki/Luminescence
https://en.wikipedia.org/wiki/Phosphorescence
https://en.wikipedia.org/wiki/Chemiluminescence
https://en.wikipedia.org/wiki/Bioluminescence
n
2016, Glowee, a French company started selling bioluminescent lights,
targeting shop fronts and municipal street signs as their main
markets.[66] France has a law that forbids retailers and offices from
illuminating their windows between 1 and 7 in the morning in order to
minimize energy consumption and pollution.[67] Glowee hoped their
product would get round this ban. They used bacteria called
Aliivibrio fischeri which glow in the dark but the maximum lifetime
of their product was three
days.[66]
https://en.wikipedia.org/wiki/Luminous_paint
1.
excerpt:
"We monitor the metabolism of aerobic bacteria by using an
oxygen sensitive fluorophore, ruthenium tris (2,2’-diprydl)
dichloride hexahydrate (RTDP) that allows us to monitor the dissolved
oxygen concentration in the nanowells. Using E.Coli K12 as a model
pathogen, we demonstrate that the detection time of E.coli can be as
fast as 35-60 minutes
the
device with smallest sized wells (1nL volume), had the largest
increase in fluorescent intensity. In comparison, the device with
100nL wells showed a slower rate of increase and those with 0.1mL
wells showed only a miniscule increase over time.
(how
about two pieces of glass stuck together? plain? 1 sanded? laser or
water jet etched? or a clutter slurry of minscule glass beads or
something)
The
powder of RTDP (ruthenium tris (2,2’-dipyridyl) dichloride
hexahydrate) was obtained from Sigma-Aldrich (#224758). Liquid
microbial growth medium, Luria Broth (LB) purchased from
Sigma-Aldrich
(#L2542)."
http://www.sigmaaldrich.com/catalog/product/aldrich/224758?lang=en®ion=US
$30
for
250mg
http://www.sigmaaldrich.com/catalog/product/sigma/l2542?lang=en®ion=US
$30
"offline
image analysis of the fluorescent intensity within the wells was
calculated by using ImageJ (NIH, rsb.info.nih.gov/ij).
E.coli
strain (OP50 and K12) was used for this study. Cells were grown
aerobically at 37 °C in LB medium (10 g tryptone, 10 g NaCl, 5 g
Yeast extract per liter). Bacterial cells in stationary phase were
harvested after overnight
incubation."
https://www.atcc.org/Products/All/10798.aspx
(K-12)
$60
http://www.sigmaaldrich.com/life-science/cell-biology/detection/learning-center/oxygen-and-nitric-oxide-probes.html
Ruthenium
based complexes have been widely used for detection and
quantification of oxygen. Fluorescence is strongly quenched by
oxygen. Its benefits are:
Long lifetime and emission around 613 nm minimizing autofluorescence background
Wide range of published applications
Ru(dpp)3(PF6) proved to be very useful for use in polystyrene membranes or beads
Excellent
stability and
photostability
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3514702/
Luminescence
lifetimes of several microseconds, in this general review, the
outlook for measurement is looking not very plain
eyeball?
http://www.solentsci.com/files/hypoxia_paper.pdf
oxygen
insensitive reference fluorophore, Nile blue chloride (222550, Sigma
Samples were excited with a 488 nm laser for Alexa Fluor 488 and
Ru(Ph2phen3)Cl2 detection and with a 635 nm laser for detection of
the Nile Blue chloride. OSBs incorporating an oxygen sensitive
luminophore (Ru(Ph2- phen3)Cl2) and two reference fluorophores (Nile
Blue chloride and Alexa Fluor 488) were produced and characterized
(Fig. 1A). The OSBs showed in their fluorescence spectra peak values
near 505 nm (Alexa Fluor 488) and 610 nm (ruthenium complex) for
excitation with a 488 nm laser, and near 655 nm (Nile Blue) for
excitation with a 635 nm laser (Fig. 1B), which indicates the
incorporation of all fluorescent molecules by the bead. The selected
ruthenium complex has a long unquenched lifetime after excitation
[31]. Presence of molecular oxygen quenches the fluorescent signal in
a diffusion-controlled manner (i.e. collisional quenching) and is
described by the SterneVolmer theory [31]. Fluorescence intensity is
therefore inversely proportional to the quencher
concentration
http://ibidi.com/fileadmin/support/literature/FL_74001_OPAL_150dpi.pdf
intra
tissue/cellular 02
measurment
2.
http://www.ncbi.nlm.nih.gov/pubmed/?term=18045388
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2007.03442.x/pdf
The
application of chromogenic media in clinical microbiology. free
review, much development is for pathogen detections that we would
want to
avoid.
http://www.shbosin.com/FileUPLoad/DownLoadFile/635736006767720821.pdf
a
review which names names of manufacturers, tables
http://www.drg-international.com/products/chromagar
appears
to be official us source, Tel: +1-973-564-7555,
ecoli
for general water quality indicator detection may allow the use of
non pathogenic bacteria EC166 or EC168, "quality control strains
ATCC 25922 and
51446"
http://www.drg-international.com/images/pdfsChroma/ecoli.pdf
$250
for freeze dried mix to 5liters, includes growth medium function with
color
http://www.atcc.org/Products/All/25922.aspx
freeze
dried biosafety 1, $60
http://www.atcc.org/Products/All/51446.aspx
freezd
dried biosafety 2,
$225
http://www.chromagar.com/
manufacturer
http://www.ncbi.nlm.nih.gov/pubmed/?term=23433363
poop
pathogen detections
Our
leader suggested this path of chromogenic detection to me.
"One
option may be to use ONPG
https://en.wikipedia.org/wiki/Ortho-Nitrophenyl-%CE%B2-galactoside
Its a sugar that turns blue when broken down by a certain enzyme,
B-galactosidase. I can easily make an Ecoli strain carrying the
B-galactosidase gene for you. Whether or not this would be a
sensitive
enough test for cell viability, I don't know.
https://www.thermofisher.com/order/catalog/product/34055
5g $87
http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Enzyme_Assay/bgalactosidaseonp.pdf
https://www.atcc.org/Products/All/37747.aspx
? $290
https://www.atcc.org/Products/All/39563.aspx
? $190
3.
based
on recent post by djwrister, a look at implementing bioluminesence
http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma-Aldrich/Datasheet/1/38719dat.pdf
30grams
Sodium chloride per liter
etc.
http://www.sigmaaldrich.com/catalog/product/sial/38719?lang=en®ion=US
$312
https://www.atcc.org/Products/All/7744.aspx
$60
26°C 78F
https://www.atcc.org/Products/All/14126.aspx
$354
26°C
78F
https://www.atcc.org/Search_Results.aspx?dsNav=Ntk:PrimarySearch%7cVibrio+splendidus%7c3%7c,Ny:True,Ro:0,N:1000552&searchTerms=Vibrio+splendidus&redir=1
similar
conditons, stronger
light?
https://www.atcc.org/Search_Results.aspx?dsNav=Ntk:PrimarySearch%7cAlteromonas+hanedai%7c3%7c,Ny:True,Ro:0,N:1000552&searchTerms=Alteromonas+hanedai&redir=1
?only
anerobic light emitter? same sigma aldritch
broth
14.0°C
https://www.atcc.org/~/media/E34D735E9B6F4F648A8CFF47D36BBC19.ashx
a
different suggested medium? other than sigma aldritch
type
https://www.atcc.org/Products/All/35081.aspx
https://www.atcc.org/Products/All/35080.aspx
https://www.atcc.org/Products/All/35082.aspx
Max
Temperature: 18.0°C
Min
Temperature: 15.0°C
$354
https://microbewiki.kenyon.edu/index.php/Photobacteria
Photobacterium
damselae: Humans can be infected with the pathogen by eating infected
fish or swimming in brackish water in which the photobacterium can
travel up the urinary tract of a person. Humans can experience organ
failure, necrotizing fasciitis, and even death. Humans can survive
between 24-72 hours with the pathogen. Antibiotics, chemotherapy, and
radiation have been used to attempt to treat the infection, but have
failed. The best recommendation is to amputate the infected area of
the body before the pathogen spreads.
[16]
http://www.disknet.com/indiana_biolab/b203.htm
For
safety, teachers might use only bioluminescent species which require
30 grams of NaCl per liter of medium. Such bacteria should not be
able to grow in any animal. Another safety precaution is to use
bacteria which can't grow at human body temperature. Many marine and
soil bacteria can't grow at such
temperatures.
https://scripps.ucsd.edu/labs/mlatz/
http://biolum.eemb.ucsb.edu/
https://en.wikipedia.org/wiki/Photobacterium
4.
"The
bacteria I was growing is called Streptomyces Coelicolor M145 ,
A(3)2. it expresses an antibiotic called actinorhodin that is blue in
a basic environment and red in an acidic environment."
Based
on Lola's information I have collected some
references:
http://www.atcc.org/Products/All/BAA-471.aspx
$354
http://www.atcc.org/~/media/D8E3FAF3DEFA4635B5023EEAFBA233F7.ashx
ATCC medium: 1877 ISP Medium 1
Tryptone (BD 211705).........5.0 g
Yeast extract................3.0 g
Agar (if necessary).........15.0 g
Distilled water..............1.0 L
Adjust
medium for final pH 7.0 - 7.2. Autoclave at 121C for 15
minutes.
http://www.atcc.org/~/media/5BC05BCB7EEA44758C09A013E3B596C3.ashx
ATCC
medium: 196 Yeast malt extract agar
Prepare Yeast Malt Extract Aqar (ISP Medium 2) (BD 277010) per
manufacturer’s
instructions.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC290581/?page=7
?
no pigment production above 30 to
32C?
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC290581/?page=3
which
type makes best
colors???
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC290581/
http://www.ncbi.nlm.nih.gov/pubmed/?term=Streptomyces+violaceoruber
http://www.ncbi.nlm.nih.gov/pubmed/?term=Streptomyces+Coelicolor+M145+A(3)2
http://www.ncbi.nlm.nih.gov/pubmed/?term=Streptomyces+Coelicolor+M145+A3(2)
https://en.wikipedia.org/wiki/Streptomyces_violaceoruber
https://en.wikipedia.org/wiki/Streptomyces_coelicolor
https://en.wikipedia.org/wiki/Streptomyces
5.
http://www.liveaquaria.com/PIC/article.cfm?aid=35
Bacterial
bloom triggered by high levels of ammonia. Nitrifying bacteria that
consume ammonia reproduce rapidly to the point that they are visible,
creating what looks like clouds of swirling, white smoke. Adding too
many fish at one time, overfeeding, over cleaning with chlorinated
water, and the use of antibiotic medications are common triggers. If
bacterial blooms occur without these triggers, it may indicate
inadequate nitrifying bacteria or biological
filtration.
https://www.microbelift.com/products/home-aquarium/bacterial-products/nite-out-ii/
"The
ammonia uptake in NOII is ≥15 mg N-NH3/hr/kg at 25°C."
For
SALT & FRESH WATER
Contains:
Nitrosomonas, Nitrospira and Nitrobactor
So
far in my tests I got rapid creation of white particles when the
"Nite out" was mixed full strength with full strength Smart
n Final ammonia, I could not get residue at the very low
concentrations suggested by the aquarium shop. Maybe the bacteria
clumped and turned white while they were trying not to die from the
ammonia concentration.
6.
decolorization:
how
about beet juice? and single strain probiotics?
so
far the multistrain probiotics did not cause decolorization in my
tests, but the unadulterated controls did decolonize... hmm,
http://www.ncbi.nlm.nih.gov/pubmed/27148725
cationic
triphenylmethane dye, crystal violet,
http://www.sigmaaldrich.com/catalog/product/sigma/c0775?lang=en®ion=US
https://en.wikipedia.org/wiki/Crystal_violet
"He
found that most Gram-positive bacteria were sensitive to the dye,
while most Gram-negative bacteria were not, and observed that the dye
tended to act as a bacteriostatic agent rather than a bactericide.it
can cause tattooing.
Streptomyces
fulvissimus CKS 7
streptomycetes
are Gram-positive, contradicts that this species eats the staining
dye?
http://www.atcc.org/Products/All/27431.aspx#documentation
http://www.ncbi.nlm.nih.gov/pubmed/19347893
seems
to produce an antibiotic that is cousin of the toxin of b cereus.
not
usually
pathogenic
http://www.ncbi.nlm.nih.gov/pubmed/21808740
triarylmethane
dyes
Aeromonas
hydrophila
http://www.atcc.org/Products/All/PTA-3751.aspx#generalinformation
http://www.ncbi.nlm.nih.gov/pubmed/16622679
Aeromonas
hydrophila
90%
within 10 h under aerobic culture condition
Crystal
Violet could be used as sole carbon source and energy source for cell
growth
http://www.ncbi.nlm.nih.gov/pubmed/26887225
Direct
Blue 151 (DB 151) and Direct Red 31 (DR 31)
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822752/
B.
cereus showed maximum decolorization of 93.37% for mixed dyes under
optimum conditions. This was followed by B. cytotoxicus, Bacillus sp.
L10, and B. flexus showing 92.77%, 86.86%, and 85% decolorization,
respectively. A study conducted by Ponraj et al.37 showed that
Bacillus sp. has high decolorizing capacity for Orange 3R. They
isolated Bacillus sp., Klebsiella sp., Salmonella sp., and
Pseudomonas sp. from textile effluent and analyzed the decolorization
of Orange 3R under optimum conditions and reported that Bacillus sp.
and Pseudomonas sp.
B.
cereus, which causes a foodborne illness similar to that of
Staphylococcus. Although most species of Bacillus are harmless
saprophytes, two species are considered medically significant:
B.anthracis and B. cereus;
B.
cereus, cheapest 60
dollars
http://www.atcc.org/Products/All/13061.aspx
http://www.atcc.org/Products/All/14579.aspx
http://www.ncbi.nlm.nih.gov/pubmed/26920535
Pseudomonas
aeruginosa strain ZM130
reactive
red-120, reactive black-5, reactive yellow-2, and reactive orange-16)
in the presence of a mixture of four different heavy metals (Cr, Zn,
Pb, Cd)
available
dried, 290$ several versions level
1
http://www.atcc.org/Products/All/47085D-5.aspx
virulence
and pathogenticity databases
http://www.mgc.ac.cn/VFs/search_VFs.htm
http://www.nmpdr.org/FIG/wiki/view.cgi
http://www.ncbi.nlm.nih.gov/pubmed/
https://www.patricbrc.org/portal/portal/patric/Home